Brain specific cell type purification


Brain cells are derived form embryonic germ layer of ectoderm (external layer) and are specialized in their functions. Neurons, ependymal cells, astrocytes, microglia, oligodendrocytes, and oligodendrocyte progenitor cells are major brain cell types, each of them contains its specific gene markers. Neurons are cell-type specific cells that release specific neurotransmitters, such as glutamate, acetylcholine, GABA, glycine, dopamine, serotonin, cannabinoid, and multiple neuropeptides. Abnormal neurotransmission and misguided neurocircuitry are involved in psychiatric and neurological diseases. Abnormal gene regulation, alternative splicing and protein modification in different cell types underline brain disease mechanisms. However, most of brain gene expression studies are carried out in bulk brain tissues with mixed cell types that could not detect subtle changes of specific neurons and glia cells. In clinical and animal brain biopsy samples, a limited needle aspiration less than 1 milligram are frequently used for detection of gene expression, genetic and epigenetic changes in a few specific type of cells.
NeuroQR bioinformatic algorithm categorizes cell-type specific protein and mRNA biomarkers for purification of different neuron and glia cell types. The brain dissociation kits contain cell dissociation enzymes, series of trituation pipettes, and reagents for effective cell dissociation for downstream cell purification of specific cell types using fluoresence-activated cell sorting (FACS) technology.

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